A new species of crayfish, Cambarus (Cambarus) eeseeohensis, is described from the Linville River of North Carolina, United States. Of the recognized members of the subgenus, it is physically most similar to Cambarus sciotensis Rhoades, 1944, which is found primarily in the Scioto River of central Ohio and New River of West Virginia and Virginia. It is easily distinguished from other recognized members of the subgenus by the strong dorsal ridges on the chelae fingers and dorsolateral impression of the propodus, second row of reduced palmer tubercles, and gape of the chelae fingers.